human nrf2 antibody Search Results


c terminal nrf2 antibody  (R&D Systems)
93
R&D Systems c terminal nrf2 antibody
Fig. 2. Keap1 protein decreases in differentiated adipocytes, while steady-state mRNA expression for <t>Nrf2</t> and Keap1 increases. 3T3-L1 cells were differentiated as described under Materials and methods and harvested on Days 0, 4, 8, 11, and 14. (A) Nrf2 and (C) Keap1 (70 kDa) protein expression was examined by immunoblotting. Black bars, full-length (FL) Nrf2 (56 kDa); gray bars, degraded (Deg) Nrf2 (40 kDa); white bars, total (Tot) Nrf2; the sums of full-length Nrf2 and degraded Nrf2 were determined to obtain relative total Nrf2 protein expression. (B) Relative steady-state Nrf2 and (D) Keap1 mRNA levels were measured by SYBRGreen qPCR and normalized to 18S rRNA. Significance was determined by one-way ANOVA Dunnett’s for protein and one-way ANOVA Bonferroni’s for mRNA; * Pr0.05 as compared to Day 0, bars indicate Pr0.05 between samples. Data are expressed as mean7SD; n¼3–4.
C Terminal Nrf2 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/c terminal nrf2 antibody/product/R&D Systems
Average 93 stars, based on 1 article reviews
c terminal nrf2 antibody - by Bioz Stars, 2026-05
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nrf2  (R&D Systems)
93
R&D Systems nrf2
Figure 3. <t>Nrf2</t> (A), Hif1-α (B), and GSK3β (C) levels in heart tissue mice to analyze oxidative stress and apoptosis after parental alcohol exposure (two different patterns of alcohol exposure, Med and Bin). Effect of postnatal treatment with EGCG on oxidative stress and apoptosis biomarkers. Nrf2: nuclear factor erythroid-2-related factor 2; Gsk3β: glycogen synthase kinase-3 beta; Hif1-α: hypoxia inducible factor 1-alpha; Med: Mediterranean group; EtOH: ethanol, Bin: binge group, EGCG: epigallocatechin-3-gallate.
Nrf2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nrf2/product/R&D Systems
Average 93 stars, based on 1 article reviews
nrf2 - by Bioz Stars, 2026-05
93/100 stars
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anti nrf2 mouse monoclonal antibody  (R&D Systems)
92
R&D Systems anti nrf2 mouse monoclonal antibody
Fig. 4. DPAA-induced time- and dose-dependent activation of MAPK signaling in cultured rat astrocytes. (A) Western blot analyses of dose- and time-dependent expressions of GFAP, HO-1, Hsp70, and <t>Nrf2</t> in cultured
Anti Nrf2 Mouse Monoclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti nrf2 mouse monoclonal antibody/product/R&D Systems
Average 92 stars, based on 1 article reviews
anti nrf2 mouse monoclonal antibody - by Bioz Stars, 2026-05
92/100 stars
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goat polyclonal  (R&D Systems)
93
R&D Systems goat polyclonal
Fig. 4. DPAA-induced time- and dose-dependent activation of MAPK signaling in cultured rat astrocytes. (A) Western blot analyses of dose- and time-dependent expressions of GFAP, HO-1, Hsp70, and <t>Nrf2</t> in cultured
Goat Polyclonal, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat polyclonal/product/R&D Systems
Average 93 stars, based on 1 article reviews
goat polyclonal - by Bioz Stars, 2026-05
93/100 stars
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human mouse rat nrf2 antibody  (R&D Systems)
N/A
The Human Mouse Rat Nrf2 Antibody from R D Systems is a mouse monoclonal antibody to Nrf2 This antibody reacts with human mouse rat The Human Mouse Rat Nrf2 Antibody has been validated for the
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human nrf2 antibody  (R&D Systems)
N/A
The Human Nrf2 Antibody from R D Systems is a goat polyclonal antibody to Nrf2 This antibody reacts with human The Human Nrf2 Antibody has been validated for the following applications Western Blot
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Fig. 2. Keap1 protein decreases in differentiated adipocytes, while steady-state mRNA expression for Nrf2 and Keap1 increases. 3T3-L1 cells were differentiated as described under Materials and methods and harvested on Days 0, 4, 8, 11, and 14. (A) Nrf2 and (C) Keap1 (70 kDa) protein expression was examined by immunoblotting. Black bars, full-length (FL) Nrf2 (56 kDa); gray bars, degraded (Deg) Nrf2 (40 kDa); white bars, total (Tot) Nrf2; the sums of full-length Nrf2 and degraded Nrf2 were determined to obtain relative total Nrf2 protein expression. (B) Relative steady-state Nrf2 and (D) Keap1 mRNA levels were measured by SYBRGreen qPCR and normalized to 18S rRNA. Significance was determined by one-way ANOVA Dunnett’s for protein and one-way ANOVA Bonferroni’s for mRNA; * Pr0.05 as compared to Day 0, bars indicate Pr0.05 between samples. Data are expressed as mean7SD; n¼3–4.

Journal: Free radical biology & medicine

Article Title: NAD(P)H:quinone oxidoreductase 1 activity reduces hypertrophy in 3T3-L1 adipocytes.

doi: 10.1016/j.freeradbiomed.2012.05.047

Figure Lengend Snippet: Fig. 2. Keap1 protein decreases in differentiated adipocytes, while steady-state mRNA expression for Nrf2 and Keap1 increases. 3T3-L1 cells were differentiated as described under Materials and methods and harvested on Days 0, 4, 8, 11, and 14. (A) Nrf2 and (C) Keap1 (70 kDa) protein expression was examined by immunoblotting. Black bars, full-length (FL) Nrf2 (56 kDa); gray bars, degraded (Deg) Nrf2 (40 kDa); white bars, total (Tot) Nrf2; the sums of full-length Nrf2 and degraded Nrf2 were determined to obtain relative total Nrf2 protein expression. (B) Relative steady-state Nrf2 and (D) Keap1 mRNA levels were measured by SYBRGreen qPCR and normalized to 18S rRNA. Significance was determined by one-way ANOVA Dunnett’s for protein and one-way ANOVA Bonferroni’s for mRNA; * Pr0.05 as compared to Day 0, bars indicate Pr0.05 between samples. Data are expressed as mean7SD; n¼3–4.

Article Snippet: We also tried the Santa Cruz N-terminal Nrf2 antibody (No. sc-13032) that gave 6–7 signals ranging from 30 to 100 kDa and another C-terminal Nrf2 antibody from R & D Systems (No. MAB3925) that gave 20þ signals ranging from 20 to 100þ kDa (data not shown).

Techniques: Expressing, Western Blot

Fig. 3. NQO1 protein increases during limited clonal expansion and postmitotic growth arrest of differentiation. 3T3-L1 cells were differentiated as described under Materials and methods and harvested on Days 0, 1, 2, 3, and 4. (A) NQO1 and (D) Keap1 protein expressions were examined by immunoblotting. (B) Relative NQO1, (C) Nrf2, and (E) Keap1 mRNA levels were measured by SYBRGreen qPCR and normalized to 18S rRNA. Significance was determined by one-way ANOVA Dunnett’s for protein and one-way ANOVA Bonferroni’s for mRNA; * Pr0.05 as compared to Day 0, bars indicate Pr0.05 between samples. Data are expressed as mean7SD; n¼3–4.

Journal: Free radical biology & medicine

Article Title: NAD(P)H:quinone oxidoreductase 1 activity reduces hypertrophy in 3T3-L1 adipocytes.

doi: 10.1016/j.freeradbiomed.2012.05.047

Figure Lengend Snippet: Fig. 3. NQO1 protein increases during limited clonal expansion and postmitotic growth arrest of differentiation. 3T3-L1 cells were differentiated as described under Materials and methods and harvested on Days 0, 1, 2, 3, and 4. (A) NQO1 and (D) Keap1 protein expressions were examined by immunoblotting. (B) Relative NQO1, (C) Nrf2, and (E) Keap1 mRNA levels were measured by SYBRGreen qPCR and normalized to 18S rRNA. Significance was determined by one-way ANOVA Dunnett’s for protein and one-way ANOVA Bonferroni’s for mRNA; * Pr0.05 as compared to Day 0, bars indicate Pr0.05 between samples. Data are expressed as mean7SD; n¼3–4.

Article Snippet: We also tried the Santa Cruz N-terminal Nrf2 antibody (No. sc-13032) that gave 6–7 signals ranging from 30 to 100 kDa and another C-terminal Nrf2 antibody from R & D Systems (No. MAB3925) that gave 20þ signals ranging from 20 to 100þ kDa (data not shown).

Techniques: Western Blot

Fig. 4. NQO1 and Keap1 proteins, but not mRNA, decreases as adipocyte differentiation progresses. 3T3-L1 cells were differentiated as described under Materials and methods and harvested on Days 4, 5, 6, 7, and 8. (A) NQO1 and (D) Keap1 protein expressions were examined by immunoblotting. (B) Relative NQO1, (C) Nrf2, and (E) Keap1 mRNA levels were measured by SYBRGreen qPCR and normalized to 18S rRNA. Significance was determined by one-way ANOVA Dunnett’s for protein and one-way ANOVA Bonferroni’s for mRNA; * Pr0.05 as compared to Day 4. Data are expressed as mean7SD; n¼3.

Journal: Free radical biology & medicine

Article Title: NAD(P)H:quinone oxidoreductase 1 activity reduces hypertrophy in 3T3-L1 adipocytes.

doi: 10.1016/j.freeradbiomed.2012.05.047

Figure Lengend Snippet: Fig. 4. NQO1 and Keap1 proteins, but not mRNA, decreases as adipocyte differentiation progresses. 3T3-L1 cells were differentiated as described under Materials and methods and harvested on Days 4, 5, 6, 7, and 8. (A) NQO1 and (D) Keap1 protein expressions were examined by immunoblotting. (B) Relative NQO1, (C) Nrf2, and (E) Keap1 mRNA levels were measured by SYBRGreen qPCR and normalized to 18S rRNA. Significance was determined by one-way ANOVA Dunnett’s for protein and one-way ANOVA Bonferroni’s for mRNA; * Pr0.05 as compared to Day 4. Data are expressed as mean7SD; n¼3.

Article Snippet: We also tried the Santa Cruz N-terminal Nrf2 antibody (No. sc-13032) that gave 6–7 signals ranging from 30 to 100 kDa and another C-terminal Nrf2 antibody from R & D Systems (No. MAB3925) that gave 20þ signals ranging from 20 to 100þ kDa (data not shown).

Techniques: Western Blot

Fig. 6. LiCl treatment increases NQO1 protein, but not NQO1 mRNA. 3T3-L1 cells were differentiated as described under Materials and methods, and treated with varying concentrations of LiCl at Day 8 and harvested on Day 11. A 40 mM NaCl was used as a vehicle control (VC). (A) NQO1 was examined by immunoblotting. (B) Relative NQO1 and (C) Nrf2 mRNA levels were measured by SYBRGreen qPCR and normalized to 18S rRNA. Significance was determined by one-way ANOVA Dunnett’s; * Pr0.05 as compared to VC for protein and mRNA. Data are expressed as mean7SD; n¼3.

Journal: Free radical biology & medicine

Article Title: NAD(P)H:quinone oxidoreductase 1 activity reduces hypertrophy in 3T3-L1 adipocytes.

doi: 10.1016/j.freeradbiomed.2012.05.047

Figure Lengend Snippet: Fig. 6. LiCl treatment increases NQO1 protein, but not NQO1 mRNA. 3T3-L1 cells were differentiated as described under Materials and methods, and treated with varying concentrations of LiCl at Day 8 and harvested on Day 11. A 40 mM NaCl was used as a vehicle control (VC). (A) NQO1 was examined by immunoblotting. (B) Relative NQO1 and (C) Nrf2 mRNA levels were measured by SYBRGreen qPCR and normalized to 18S rRNA. Significance was determined by one-way ANOVA Dunnett’s; * Pr0.05 as compared to VC for protein and mRNA. Data are expressed as mean7SD; n¼3.

Article Snippet: We also tried the Santa Cruz N-terminal Nrf2 antibody (No. sc-13032) that gave 6–7 signals ranging from 30 to 100 kDa and another C-terminal Nrf2 antibody from R & D Systems (No. MAB3925) that gave 20þ signals ranging from 20 to 100þ kDa (data not shown).

Techniques: Control, Western Blot

Fig. 7. Sulforaphane increases NQO1 protein and mRNA levels. 3T3-L1 cells were differentiated as described under Materials and methods, and treated with varying concentrations of sulforaphane (SFN). A 0.3% DMSO was used as a VC. (A) NQO1 was examined by immunoblotting. (B) Relative NQO1 and (C) Nrf2 mRNA levels were measured by SYBRGreen qPCR and normalized to 18S rRNA. Significance was determined by one-way ANOVA Dunnett’s; * Pr0.05 as compared to VC for protein and mRNA. Data are expressed as mean7SD; n¼3.

Journal: Free radical biology & medicine

Article Title: NAD(P)H:quinone oxidoreductase 1 activity reduces hypertrophy in 3T3-L1 adipocytes.

doi: 10.1016/j.freeradbiomed.2012.05.047

Figure Lengend Snippet: Fig. 7. Sulforaphane increases NQO1 protein and mRNA levels. 3T3-L1 cells were differentiated as described under Materials and methods, and treated with varying concentrations of sulforaphane (SFN). A 0.3% DMSO was used as a VC. (A) NQO1 was examined by immunoblotting. (B) Relative NQO1 and (C) Nrf2 mRNA levels were measured by SYBRGreen qPCR and normalized to 18S rRNA. Significance was determined by one-way ANOVA Dunnett’s; * Pr0.05 as compared to VC for protein and mRNA. Data are expressed as mean7SD; n¼3.

Article Snippet: We also tried the Santa Cruz N-terminal Nrf2 antibody (No. sc-13032) that gave 6–7 signals ranging from 30 to 100 kDa and another C-terminal Nrf2 antibody from R & D Systems (No. MAB3925) that gave 20þ signals ranging from 20 to 100þ kDa (data not shown).

Techniques: Western Blot

Figure 3. Nrf2 (A), Hif1-α (B), and GSK3β (C) levels in heart tissue mice to analyze oxidative stress and apoptosis after parental alcohol exposure (two different patterns of alcohol exposure, Med and Bin). Effect of postnatal treatment with EGCG on oxidative stress and apoptosis biomarkers. Nrf2: nuclear factor erythroid-2-related factor 2; Gsk3β: glycogen synthase kinase-3 beta; Hif1-α: hypoxia inducible factor 1-alpha; Med: Mediterranean group; EtOH: ethanol, Bin: binge group, EGCG: epigallocatechin-3-gallate.

Journal: Antioxidants (Basel, Switzerland)

Article Title: Effect of Postnatal Epigallocatechin-Gallate Treatment on Cardiac Function in Mice Prenatally Exposed to Alcohol.

doi: 10.3390/antiox12051067

Figure Lengend Snippet: Figure 3. Nrf2 (A), Hif1-α (B), and GSK3β (C) levels in heart tissue mice to analyze oxidative stress and apoptosis after parental alcohol exposure (two different patterns of alcohol exposure, Med and Bin). Effect of postnatal treatment with EGCG on oxidative stress and apoptosis biomarkers. Nrf2: nuclear factor erythroid-2-related factor 2; Gsk3β: glycogen synthase kinase-3 beta; Hif1-α: hypoxia inducible factor 1-alpha; Med: Mediterranean group; EtOH: ethanol, Bin: binge group, EGCG: epigallocatechin-3-gallate.

Article Snippet: Glycogen synthase kinase-3 beta (Gsk3β) (ref. ab227208, 46 kDa) and B-type natriuretic peptide (BNP) (ref. ab236101, 15 kDa) were obtained from Abcam (Madrid, Spain); Nrf2 (ref. af3925, 90 kDa) from R&D systems (Minneapolis, MN, USA); the hypoxia inducible factor 1-Alpha (Hif1-α) (ref. sc-13515, 130 kDa), β-cell lymphoma 2 (Bcl-2) (ref. sc-7382), glutathione peroxidase (GPx) (ref. sc-133160), catalase (ref. sc-271803), and troponin I (ref. sc-133117, 29 kDa) from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA); superoxide dismutase 2 (SOD-2) (ref.13141) and Bcl-2-like protein 4 (Bax) (ref. 2771) were from Cell Signaling (Danvers, MA, USA); alpha-tubulin (ref. T8203, dilution 1:2000, 50 kDa) and anti-rabbit IgG secondary antibody (ref. A0545, dilution 1:2000) from Sigma-Aldrich (Sant Louis, MO, USA); goat anti-mouse IgG (ref. G21040, dilution 1:10,000) from Thermo Fisher Technologies (Waltham, MA, USA).

Techniques:

Fig. 4. DPAA-induced time- and dose-dependent activation of MAPK signaling in cultured rat astrocytes. (A) Western blot analyses of dose- and time-dependent expressions of GFAP, HO-1, Hsp70, and Nrf2 in cultured

Journal: Toxicological sciences : an official journal of the Society of Toxicology

Article Title: Diphenylarsinic Acid Induced Activation of Cultured Rat Cerebellar Astrocytes: Phosphorylation of Mitogen-Activated Protein Kinases, Upregulation of Transcription Factors, and Release of Brain-Active Cytokines.

doi: 10.1093/toxsci/kfv310

Figure Lengend Snippet: Fig. 4. DPAA-induced time- and dose-dependent activation of MAPK signaling in cultured rat astrocytes. (A) Western blot analyses of dose- and time-dependent expressions of GFAP, HO-1, Hsp70, and Nrf2 in cultured

Article Snippet: In the present study, the following primary antibodies were used: Anti-GFAP mouse monoclonal antibody [#3670 (clone GA5), Cell Signaling Technology (CST), MA, USA]; anti-HO-1 rabbit polyclonal antibody (ab13243, Abcam, Cambridge, UK); anti-Hsp70 mouse monoclonal antibody [SPA-810 (clone C92F3A-5), Enzo Life Sciences, PA, USA]; anti-Nrf2 mouse monoclonal antibody [MAB3925 (clone 383727), R&D Systems, MN, USA]; anti-phospho-ERK1/2 rabbit monoclonal antibody [#4370 (clone D13.14.4E), CST]; anti-ERK1/2 mouse monoclonal antibody [#9107 (clone 3A7), CST]; anti-phospho-p38MAPK rabbit monoclonal antibody [#4511 (clone D3F9), CST]; anti-p38MAPK rabbit monoclonal antibody [#8690 (clone D13E1), CST]; anti-phospho-SAPK/JNK rabbit monoclonal antibody [#4668 (clone 81E11), CST]; anti-SAPK/JNK rabbit monoclonal antibody [#9258 (clone 56G8), CST]; anti-phospho-CREB rabbit monoclonal antibody [#9198 (clone 87G3), CST]; anti-CREB at U niversity of C alifornia, San D iego on January 16, 2016 http://toxsci.oxfordjournals.org/ D ow nloaded from 9 mouse monoclonal antibody [#9104 (clone 86B10), CST]; anti-phospho-c-Jun rabbit monoclonal antibody [#3270 (clone D47G9), CST]; anti-c-Jun rabbit monoclonal antibody [#9165 (clone 60A8), CST]; anti-phospho-c-Fos rabbit monoclonal antibody [#5348 (clone D82C12), CST]; anti-c-Fos rabbit monoclonal antibody [#2250 (clone 9F6), CST]; anti-β-actin mouse monoclonal antibody [A1978 (clone AC-15), Sigma-Aldrich]; and anti-β-actin rabbit monoclonal antibody [#4970 (clone 13E5), CST].

Techniques: Activation Assay, Cell Culture, Western Blot